rat cardiac fibroblasts rcf  (Cell Applications Inc)

Journal: Current Issues in Molecular Biology

Article Title: Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

doi: 10.3390/cimb47100798

Figure Lengend Snippet: Venn diagram of MG-EWE active ingredient targets and proteins related to IL-6 production in cardiac fibroblasts.

Article Snippet: Rat Cardiac Fibroblast Cells (CP-R074) and Rat Cardiac Fibroblast Cell Complete Medium (CM-R074) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

doi: 10.3390/cimb47100798

Figure Lengend Snippet: The schematic diagram illustrates the molecular mechanism through which MG suppresses IL-6 production in cardiac fibroblasts (CFs): in the isoproterenol (ISO)-induced CFs transdifferentiation model, MG-EWE and its active components Laurolitsine and Hecogenin can inhibit the abnormal activation of the ADRB2/JNK/c-Jun signaling pathway. When ISO binds to the ADRB2 receptor on the cell membrane surface of CFs, it initiates a downstream signaling cascade. Activation of this receptor leads to the phosphorylation of key signaling molecules, including JNK1/2/3 and c-Jun, thereby activating the JNK/c-Jun signaling pathway. The activated JNK1/2/3 translocates from the cytoplasm to the nucleus and further phosphorylates the transcription factor c-Jun within the nucleus, enhancing its transcriptional activity. This ultimately promotes the transcription of the IL-6 gene and increases its protein expression. Laurolitsine functions by inhibiting the activation of ADRB2 on the CFs cell membrane surface, while Hecogenin inhibits the phosphorylation of JNK, thereby blocking the subsequent phosphorylation of c-Jun. Together, these actions result in the joint inhibition of IL-6 production.

Article Snippet: Rat Cardiac Fibroblast Cells (CP-R074) and Rat Cardiac Fibroblast Cell Complete Medium (CM-R074) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques: Activation Assay, Membrane, Phospho-proteomics, Activity Assay, Expressing, Blocking Assay, Inhibition